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Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato PAB
Amaral,Daniel Oliveira Jordão do; Lima,Marleide Magalhães de Andrade; Resende,Luciane Vilela; Silva,Márcia Vanusa da.
The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expressed. A total of 307 clones was identified in two subtractive libraries, which allowed the isolation of several defense-related genes that play roles in different mechanisms of plant resistance to phytopathogens. Genes with unknown roles were also isolated from the two libraries, which indicates the possibility of identifying new genes not yet reported in studies of stress/defense response. The SSH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; CDNA libraries; Complementary DNA; Gene expression; Salicylic acids; Suppression subtractive hybridization.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800010
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Germination of cotton cultivar seeds under water stress induced by polyethyleneglycol-6000 Scientia Agricola
Meneses,Carlos Henrique Salvino Gadelha; Bruno,Riselane de Lucena Alcântara; Fernandes,Pedro Dantas; Pereira,Walter Esfrain; Lima,Leonardo Henrique Guedes de Morais; Lima,Marleide Magalhães de Andrade; Vidal,Márcia Soares.
The physiological quality of cotton cultivar seeds (Gossypium hirsutum var. latifolium L.) was evaluated in laboratory by the simulation of water potentials with polyethyleneglycol-6000 (0.0; -0.2; -0.4; -0.6; -0.8 and -1.0 MPa), at 25ºC using germitest paper as substrate. A completely randomized design in a 4 × 6 factorial scheme with four replications of 50 seeds each was used. The studied variables were: germination percentage, first count of germination, germination velocity index, accelerated aging in water, electrical conductivity, humidity, vigor classification, radicle length and radicle/shoot length ratio. The effect of water stress on seed viability and on plantlet vigor was severe at potentials below -0.4 MPa. The 'CNPA 187 8H' cultivar was the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum var. latifolium L.; PEG-6000; Vigor; Water potential.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000200001
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